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Protein Biotech Platform

Multiple cells for protein expression

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Our laboratory has established a series of protein expression systems, including E.coli, yeast, insect cells, mammalian cells, and the Expi CHO high-expression system, and developed chemical engineering methods for generating long-acting proteins such as lipidation. We have successfully produced over a hundred high-purity protein products with excellent biological activity. 

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  1. Insect cells

  2. Mammalian cells

  3. E.coli

  4. Yeast

All enquiries are welcome to Jiang Xue (xue0129@hku.hk) at 852 3502 2780

Anaerobic Chamber (DWS DG250)

ÄKTA_Explorer™_with_Fraction_Collect

ÄKTAexplorer™ chromotography systems are designed for fast development and optimization of any biomolecular purification, using one working platform for all techniques and samples. The method wizard and optimized system configurations, allow rapid media screening, method scouting, method optimization, and scale-up experiments

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  1. Fast, simple start to every purification.

  2. Simple column selection suited to each purification.

  3. Fast, systematic method optimization.

  4. No manual titration of buffers.

  5. Flexibility from laboratory to production.

All enquiries are welcome to Jiang Xue (xue0129@hku.hk) at 852 3502 2780

RALF Autoclavable Benchtop Bioreactor BiOENGiNEERiNG (Switzerland)

RALF Autoclavable Benchtop Bioreactor Bi

  1. Bioengineering RALF for Cell Culture or Microbial Fermentation.

  2. Rounded-bottom autoclavable laboratory fermentor/bioreactor.

  3. Unlimited options for pumps and gas lines.

  4. Can do cell and microbial culture in the same reactor due to free configuration of gas lines.

  5. Comes with the most comprehensive bioprocess management software: BioSCADA Ralf includes complete process automation, recipe features, audit trail, access level management, batch reporting, and various other advanced features.

  6. Free I/O modules for external devices such as exhalyzers, VOC, probes, etc.

All enquiries are welcome to Jiang Xue (xue0129@hku.hk) at 852 3502 2780

Non-contacting Protein Dispenser

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  1. Allow to dispense samples in a non-contacting manner.

  2. Allow to dispense samples quantitatively in a “Step-and-Repeat” mode with a dispensing volume from 1.3 nL- 2 μL as a single drop.

  3. Multi-mode dispensing in terms of dot dispensing and line dispensing with automatic wash, aspiration and dispense.

  4. Suitable for applications including Lateral Flow, Immunoblot, and Flow Through.

All enquiries are welcome to Jiang Xue (xue0129@hku.hk) at 852 3502 2780

PurePep® Chorus Multiple Peptide Synthesizer

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The design power of the proprietary PurePep Pathway (valve block system) results in zero dead volumes, no cross-contamination, and worry-free performance that lasts for years. No cross-contamination, and no failure due to harsh peptide synthesis chemicals. With the trusted PurePep Pathway inside PurePep Chorus, you have reliable peptide purity every time.​

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  1. Allow to dispense samples in a non-contacting manner.

  2. Allow to dispense samples quantitatively in a “Step-and-Repeat” mode with a dispensing volume from 1.3 nL- 2 μL as a single drop.

  3. Multi-mode dispensing in terms of dot dispensing and line dispensing with automatic wash, aspiration and dispense.

  4. Suitable for applications including Lateral Flow, Immunoblot, and Flow Through.

All enquiries are welcome to Dicky (dickylkm@hku.hk) 

Yeast Two-hybrid (Y2H) Screening

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​Yeast two-hybrid (Y2H) screening is a mature technique to discover novel protein interactions and maps the precise binding regions between two proteins. Our service includes:

  1. Cloning: Subclone gene of interest into Y2H vector and transform into yeast.

  2. Testing for “bait” auto-activation.

  3. Screening: Perform large scale (pre-made or custom-made) library screen.

  4. Screening: Phenotypic analysis (up to 4 reporter genes are used).

  5. Screening: Recover plasmids from yeast and transform into E.coli.

  6. Screening: Determine identity of “prey” by sequencing.

  7. Validation: Retransform yeast with recovered plasmids to verify interactions.

All enquiries are welcome to Jiang Xue (xue0129@hku.hk) at 852 3502 2780

Viral Services

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​We offer a broad range of viral expression system (Adenovirus, Lentivirus and Adeno-associated virus) for gene delivery.

  1. Subcloning your gene of interest into related vector.

  2. Large-scale preparation of virus.

  3. Determination of the viral titer.

  4. Technical Support.

All enquiries are welcome to Jiang Xue (xue0129@hku.hk) at 852 3502 2780

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