香港大學生物醫藥技術國家重點實驗室
State Key Laboratory of Pharmaceutical Biotechnology
The University of Hong Kong
In-vitro Analytical & Assay
XF Extracellular Flux Analyzers (Seahorse)
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In vitro measurement of oxygen consumption, glycolysis, carbon dioxide, mitochondrial function, and fatty acid oxidation.
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Effect of oxidative stress on mitochondrial functions.
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Test the capacity of cells to maintain ATP level upon treatment with mitochondrial respiration inhibitor or glycolytic inhibitor.
All enquiries are welcome to Baile WANG (blwong@connect.hku.hk)
BS120 Automatic Biochemical Analyzer (Mindray)
A bench-top analyzer for clinical biochemical analysis and biochemical reagents development.
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Automatic dilution system.
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37 °C incubation.
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Test speed: 100 test/hour.
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8 different wavelengths.
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Minimum reaction volume: 180 μl.
All enquiries are welcome to Pengcheng Zhou (zhoupc@hku.hk) at 28315525
Synergy H1 Hybrid Microplate Reader (BioTek)
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Excellent plate reader: reliable, fast, good sensitivity.
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Monochromator absorbance.
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Full-light luminescence.
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Fluorescence polarization.
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Time resolved fluorescence.
All enquiries are welcome to Pengcheng Zhou (zhoupc@hku.hk) at 28315525
Anaerobic Chamber (DWS DG250)
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The workstation is used to process, culture and examine anaerobic samples without exposure to atmospheric oxygen.
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The workstation can accommodate 270 x 90mm Petri dishes.
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Temperature control: 5°C-45°C.
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The user portholes act as two mini-airlocks so the users can transfer plates at the same time as entering/exiting the workstation.
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The workstation is available with automatic sleeve gassing system operated by foot-switch so the users can ensure the correct conditions and use gas supplies efficiently.
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Gas supplies: 100% N2 and mix gas (10% H2 10% CO2 80% N2).
All enquiries are welcome to Jianyu Yao (jyyao@hku.hk) at 53720954
Gas Chromatography–Mass Spectrometry (TRACE 1300/ISQ Single Quadrupole)
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Separating different components of a mixture that can be vaporized without decomposition (environmental science, industrial chemicals and life science).
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Full Scan/SIM simultaneous within sample injection for confirmation and improved quantitation in the same analysis.
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AutoSIM for quick and automated method development.
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Inert ion source and sample flow path for highest chromatographic integrity.
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Quick column change is enabled without venting the mass spectrometer.
All enquiries are welcome to Pengcheng Fan (pcfan@hku.hk) at 39179257
High-pressure Liquid Chromatography(Agilent 1100 series)
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Rapid separation the components of a mixture from various biological samples.
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Pumps with flow control are available for automatic gradient delivery.
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Autosampler is ready for rapid, accurate, and precise injections.
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Ultraviolet (UV) detector allows the measurement of sample to absorb light at different wavelengths.
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Provides high-sensitivity fluorescence detection with wavelength range up to 900 nm without sacrificing sensitivity.
All enquiries are welcome to Pengcheng Fan (pcfan@hku.hk) at 39179257
Multi-Channel Oxytherm System (Hansatech)
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For real-time measurement of oxygen signals in isolated mitochondria and cells
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User-friendly O2 data acquisition and system configuration software /li>
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8 channels which enables simultaneous measurement of multiple samples
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Suitable for examination of mitochondria respiration and function
All enquiries are welcome to Baile WANG (blwong@connect.hku.hk)
Yeast Two-hybrid (Y2H) Screening
Yeast two-hybrid (Y2H) screening is a mature technique to discover novel protein interactions and maps the precise binding regions between two proteins. Our service includes:
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Cloning: Subclone gene of interest into Y2H vector and transform into yeast.
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Testing for “bait” auto-activation.
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Screening: Perform large scale (pre-made or custom-made) library screen.
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Screening: Phenotypic analysis (up to 4 reporter genes are used).
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Screening: Recover plasmids from yeast and transform into E.coli.
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Screening: Determine identity of “prey” by sequencing.
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Validation: Retransform yeast with recovered plasmids to verify interactions.
All enquiries are welcome to Chi-Ming Wong (wispwong@gmail.com)
Viral Services
We offer a broad range of viral expression system (Adenovirus, Lentivirus and Adeno-associated virus) for gene delivery.
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Subcloning your gene of interest into related vector.
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Large-scale preparation of virus.
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Determination of the viral titer.
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Technical Support.
All enquiries are welcome to Huang Zhe (huangzhe@hku.hk) at 39179763